- Pfad:
-
Tabelle: Tabellen der Antritts-Gelder und jährlichen Beyträge für Männer von 20 bis 60 Jahren, [...]
Zeitschrift
- Titel:
- Biological chemistry
- Erschienen:
-
Berlin [u.a.]: de Gruyter
- Fußnote:
- Gesehen am 11.02.10
- Umfang:
- Online-Ressource
- ISSN:
- 1437-4315
- ZDB-ID:
-
1466062-3
- VÖBB-Katalog:
- 15134825
- Frühere Titel:
- Biological chemistry Hoppe-Seyler
- Schlagworte:
- Biochemie ; Physiologische Chemie ; Molekularbiologie ; Zeitschrift
- ZLB-Systematik:
- Naturwissenschaften
- Sammlung:
- Naturwissenschaften
- Copyright:
- Rechte vorbehalten
- Zugriffsberechtigung:
- Eingeschränkter Zugang mit Nutzungsbeschränkungen
- Titel:
- Biological chemistry
- Erschienen:
-
Berlin [u.a.]: de Gruyter
- Fußnote:
- Gesehen am 11.02.10
- Umfang:
- Online-Ressource
- ISSN:
- 1437-4315
- ZDB-ID:
-
1466062-3
- VÖBB-Katalog:
- 15134825
- Frühere Titel:
- Biological chemistry Hoppe-Seyler
- Schlagworte:
- Biochemie ; Physiologische Chemie ; Molekularbiologie ; Zeitschrift
- ZLB-Systematik:
- Naturwissenschaften
- Sammlung:
- Naturwissenschaften
- Copyright:
- Rechte vorbehalten
- Zugriffsberechtigung:
- Eingeschränkter Zugang mit Nutzungsbeschränkungen
Aufsatz
- Titel:
- Apolipoprotein E-containing lipoproteins and their extracellular interactions with LRP1 affect LPS-induced inflammation
- Erschienen:
-
Berlin [u.a.]: de Gruyter, 2024
- Sprache:
- Englisch
- Zusammenfassung:
- Abstract: The linkage between low-density lipoprotein receptor-related protein (LRP) 1-mediated metabolism of apolipoprotein (apo) E-containing lipoproteins (apoE-LP) and the lipopolysaccharide (LPS)-induced inflammatory response contributes to the pathogenesis of sepsis; however, the underlying mechanisms are unclear. Therefore, in this study, the effects of apoE-LP and their constituents on the mRNA expression of interleukin (IL)-6 and LRP1 were evaluated using a culture system of human fibroblasts supplemented with LPS and apoE-containing emulsion particles (apoE-EP). The affinity of apoE-LP for LPS was examined using the interaction between fluorescence-labeled LPS and serum lipoprotein fractions. LPS-induced inflammation significantly upregulated the mRNA expression of IL-6 and LRP1. This upregulation was markedly suppressed by pre-incubation of LPS with apoE-EP or its constituents (apoE or EP). The suppressive effect of apoE-EP on IL-6 upregulation was attenuated in the presence of lactoferrin, an inhibitor of LRP1. The prepared apoE-EP and serum triglyceride-rich lipoproteins showed significant affinity for LPS. However, these affinities appeared to be lower than expected based on the extent to which IL-6 upregulation was suppressed by pre-incubation of LPS with apoE-EP. Overall, these results indicate that LPS-induced inflammation may be regulated by 1) the LPS-neutralizing effect of apoE-LP, 2) anti-inflammatory effect of apoE, and 3) LRP1-mediated metabolic pathways.
- Umfang:
- Online-Ressource
- Fußnote:
- Kein Open Access
- Archivierung/Langzeitarchivierung gewährleistet
- Schlagworte:
- sepsis ; IL-6 ; anti-inflammatory ; triglyceride-rich lipoproteins ; VLDL ; neutralization effect
- ZLB-Systematik:
- Naturwissenschaften
- Sonstiges
- Sammlung:
- Naturwissenschaften
- Sonstiges
- Copyright:
- Rechte vorbehalten
- Zugriffsberechtigung:
- Eingeschränkter Zugang mit Nutzungsbeschränkungen
- Titel:
- Apolipoprotein E-containing lipoproteins and their extracellular interactions with LRP1 affect LPS-induced inflammation
- Erschienen:
-
Berlin [u.a.]: de Gruyter, 2024
- Sprache:
- Englisch
- Zusammenfassung:
- Abstract: The linkage between low-density lipoprotein receptor-related protein (LRP) 1-mediated metabolism of apolipoprotein (apo) E-containing lipoproteins (apoE-LP) and the lipopolysaccharide (LPS)-induced inflammatory response contributes to the pathogenesis of sepsis; however, the underlying mechanisms are unclear. Therefore, in this study, the effects of apoE-LP and their constituents on the mRNA expression of interleukin (IL)-6 and LRP1 were evaluated using a culture system of human fibroblasts supplemented with LPS and apoE-containing emulsion particles (apoE-EP). The affinity of apoE-LP for LPS was examined using the interaction between fluorescence-labeled LPS and serum lipoprotein fractions. LPS-induced inflammation significantly upregulated the mRNA expression of IL-6 and LRP1. This upregulation was markedly suppressed by pre-incubation of LPS with apoE-EP or its constituents (apoE or EP). The suppressive effect of apoE-EP on IL-6 upregulation was attenuated in the presence of lactoferrin, an inhibitor of LRP1. The prepared apoE-EP and serum triglyceride-rich lipoproteins showed significant affinity for LPS. However, these affinities appeared to be lower than expected based on the extent to which IL-6 upregulation was suppressed by pre-incubation of LPS with apoE-EP. Overall, these results indicate that LPS-induced inflammation may be regulated by 1) the LPS-neutralizing effect of apoE-LP, 2) anti-inflammatory effect of apoE, and 3) LRP1-mediated metabolic pathways.
- Umfang:
- Online-Ressource
- Fußnote:
- Kein Open Access
- Archivierung/Langzeitarchivierung gewährleistet
- Schlagworte:
- sepsis ; IL-6 ; anti-inflammatory ; triglyceride-rich lipoproteins ; VLDL ; neutralization effect
- ZLB-Systematik:
- Naturwissenschaften
- Sonstiges
- Sammlung:
- Naturwissenschaften
- Sonstiges
- Copyright:
- Rechte vorbehalten
- Zugriffsberechtigung:
- Eingeschränkter Zugang mit Nutzungsbeschränkungen
