- Pfad:
-
Candidate reference measurement procedure based on isotope dilution-two dimensional-liquid chromatography-tandem mass spectrometry for the quantification of androstenedione in human serum and plasma
Dateien
Externe Ressourcen
Zeitschrift
- Titel:
- Clinical chemistry and laboratory medicine
- Erschienen:
-
Berlin [u.a.]: De Gruyter
- Fußnote:
- Gesehen am 22.6.2022
- Archivierung/Langzeitarchivierung gewährleistet
- 82+C!EBSCO-Split(17-11-09)
- Umfang:
- Online-Ressource
- ISSN:
- 1437-4331
- ZDB-ID:
-
1492732-9
- VÖBB-Katalog:
- 15133150
- Schlagworte:
- Zeitschrift
- ZLB-Systematik:
- Naturwissenschaften
- Medizin
- Dewey-Dezimalklassifikation:
- 610 Medizin
- 540 Chemie
- Sammlung:
- Naturwissenschaften
- Medizin
- Copyright:
- Rechte vorbehalten
- Zugriffsberechtigung:
- Eingeschränkter Zugang mit Nutzungsbeschränkungen
Aufsatz
- Titel:
- Candidate reference measurement procedure based on isotope dilution-two dimensional-liquid chromatography-tandem mass spectrometry for the quantification of androstenedione in human serum and plasma
- Erschienen:
-
Berlin [u.a.]: De Gruyter, 2026
- Sprache:
- Englisch
- Zusammenfassung:
- Objectives: Androstenedione (ASD) is a biomarker used in the diagnosis of hyperandrogenism and adrenal hyperplasia. Therefore, accurate measurement of serum ASD is pivotal in clinical settings. We aimed to develop a novel highly selective reference measurement procedure (RMP) based on isotope dilution-two dimensional-liquid chromatography-tandem mass spectrometry (ID-2D-LC-MS/MS) for the quantification of ASD in human serum/plasma. Methods: To achieve high selectivity and sensitivity, a two-dimensional heart-cut LC approach for LC-MS/MS and a supported liquid extraction sample preparation protocol were employed. Matrix effects were evaluated through a post-column infusion experiment and comparison of standard line slopes. Precision and accuracy were determined via a multi-day validation experiment. Reproducibility was assessed by comparing results from two independent laboratories, and measurement uncertainty (MU) was evaluated in compliance with current guidelines. Results: Our novel RMP proved effective for measuring ASD concentrations ranging from 0.00800 ng/mL to 12.0 ng/mL (0.0279 nmol/L to 41.9 nmol/L) and demonstrated matrix-independence. The relative mean bias varied between 0.6 and 2.2 % across different matrices and concentration levels. Intermediate precision was observed to be between 1.2 and 1.9 %. The expanded measurement uncertainty for ASD target value assignment ranged from 1.7 to 2.5 %, irrespective of sample concentration. Equivalence to the JCTLM-listed RMP was evaluated through a method comparison study, revealing a high degree of agreement (r≥0.997). Additionally, by comparing results from two independent laboratories, the transferability and reproducibility of the RMP were confirmed. Conclusions: This isotope dilution two-dimensional LC-MS/MS method can be used for the evaluation and standardization of routine assays and for measuring individual patient samples, ensuring traceability.
- Umfang:
- Online-Ressource
- Fußnote:
- Open Access
- Archivierung/Langzeitarchivierung gewährleistet
- Schlagworte:
- androstenedione ; isotope dilution-liquid chromatography-tandem mass spectrometry ; reference measurement procedure ; qNMR characterization ; SI units ; traceability
- ZLB-Systematik:
- Naturwissenschaften
- Medizin
- Sammlung:
- Naturwissenschaften
- Medizin
- Copyright:
- CC BY
- Zugriffsberechtigung:
- Freier Zugang
