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6-Methoxyflavone improves anxiety, depression, and memory by increasing monoamines in mice brain: HPLC analysis and in silico studies / Arif, Mehreen (CC BY)

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fullscreen : 6-Methoxyflavone improves anxiety, depression, and memory by increasing monoamines in mice brain: HPLC analysis and in silico studies / Arif, Mehreen (CC BY)

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6-Methoxyflavone improves anxiety, depression, and memory by increasing monoamines in mice brain: HPLC analysis and in silico studies / Arif, Mehreen (CC BY)

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6-Methoxyflavone improves anxiety, depression, and memory by increasing monoamines in mice brain: HPLC analysis and in silico studies / Arif, Mehreen (CC BY)

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Zeitschrift

Titel:
Open chemistry
Erschienen:
Berlin: de Gruyter
Fußnote:
Gesehen am 23.06.15
Open Access
Namensnennung 4.0 International
Umfang:
Online-Ressource
ISSN:
2391-5420
ZDB-ID:
2825411-9 ZDB
VÖBB-Katalog:
35314079
Frühere Titel:
Central European journal of chemistry
Schlagworte:
Zeitschrift
ZLB-Systematik:
Naturwissenschaften
Sammlung:
Naturwissenschaften
Copyright:
Rechte vorbehalten
Zugriffsberechtigung:
Freier Zugang
Titel:
Open chemistry
Erschienen:
Berlin: de Gruyter
Fußnote:
Gesehen am 23.06.15
Open Access
Namensnennung 4.0 International
Umfang:
Online-Ressource
ISSN:
2391-5420
ZDB-ID:
2825411-9 ZDB
VÖBB-Katalog:
35314079
Frühere Titel:
Central European journal of chemistry
Schlagworte:
Zeitschrift
ZLB-Systematik:
Naturwissenschaften
Sammlung:
Naturwissenschaften
Copyright:
Rechte vorbehalten
Zugriffsberechtigung:
Freier Zugang

Aufsatz

Verfasser:
Arif, Mehreen
Ur Rehman, Naeem
Anjum, Irfan
Rauf, Khalid
Alotaibi, Amal
Alhmidani, Ghala
Titel:
6-Methoxyflavone improves anxiety, depression, and memory by increasing monoamines in mice brain: HPLC analysis and in silico studies
Erschienen:
Berlin: de Gruyter, 2024
Sprache:
Englisch
Zusammenfassung:
Abstract: 6-Methoxyflavone (6-MOF) is a flavonoid that has been reported to be a GABA-A receptor agonist and reverses cisplatin-induced hyperalgesia and allodynia. Considering the varied neuropharmacological profile of 6-MOF, this study was intended to determine the pharmacological effects of 6-MOF on locomotion, anxiety, novel object recognition (NOR), depression, spatial memory, socialization behavior, nest-building behavior, and depression in various groups of mice. Selected groups of mice were injected with 25, 50, and 75 mg/kg 6-MOF. Using HPLC-UV, the frontal cortex, striatum, and hippocampus of the sacrificed mice were analyzed for the levels of vitamin C, dopamine, serotonin, noradrenaline, adenosine, and its metabolites. Statistical analysis showed significant results in socialization behavior and elevated plus maze with 75 mg/kg. In Y-maze, NOR 6-MOF showed significant results at all three doses, while in tail suspension test (TST), 50 and 75 mg/kg showed significant results; however, no statistical significance was observed in nest-building behavior; 50 and 75 mg/kg 6-MOF showed significant results in the Morris water maze. 6-MOF raised vitamin C levels in the frontal cortex and hippocampus. Serotonin, dopamine, and nor-adrenaline levels were raised in the hippocampus and striatum. It has also imparted region-specific neuroprotection by improving adenosine and its metabolite levels. In silico studies performed using PyRx have shown that the minimum binding energy of 6-MOF with antioxidant enzyme is −7.1 k/cal/mol. The binding energy showed that 6-MOF was successfully docked with an anti-oxidant enzyme. In conclusion, in silico and behavioral studies showed that 6-MOF can be a potential candidate for the treatment of cognitive decline, anxiety, and depression.
Umfang:
Online-Ressource
Fußnote:
Open Access
Archivierung/Langzeitarchivierung gewährleistet
Schlagworte:
serotonin ; dopamine ; noradrenaline ; 6-methoxyflavone ; adenosine
ZLB-Systematik:
Naturwissenschaften
Sonstiges
URN:
urn:nbn:de:101:1-2410261535214.298236181298
Sammlung:
Naturwissenschaften
Sonstiges
Copyright:
CC BY
Zugriffsberechtigung:
Freier Zugang
Verfasser:
Arif, Mehreen
Ur Rehman, Naeem
Anjum, Irfan
Rauf, Khalid
Alotaibi, Amal
Alhmidani, Ghala
Titel:
6-Methoxyflavone improves anxiety, depression, and memory by increasing monoamines in mice brain: HPLC analysis and in silico studies
Erschienen:
Berlin: de Gruyter, 2024
Sprache:
Englisch
Zusammenfassung:
Abstract: 6-Methoxyflavone (6-MOF) is a flavonoid that has been reported to be a GABA-A receptor agonist and reverses cisplatin-induced hyperalgesia and allodynia. Considering the varied neuropharmacological profile of 6-MOF, this study was intended to determine the pharmacological effects of 6-MOF on locomotion, anxiety, novel object recognition (NOR), depression, spatial memory, socialization behavior, nest-building behavior, and depression in various groups of mice. Selected groups of mice were injected with 25, 50, and 75 mg/kg 6-MOF. Using HPLC-UV, the frontal cortex, striatum, and hippocampus of the sacrificed mice were analyzed for the levels of vitamin C, dopamine, serotonin, noradrenaline, adenosine, and its metabolites. Statistical analysis showed significant results in socialization behavior and elevated plus maze with 75 mg/kg. In Y-maze, NOR 6-MOF showed significant results at all three doses, while in tail suspension test (TST), 50 and 75 mg/kg showed significant results; however, no statistical significance was observed in nest-building behavior; 50 and 75 mg/kg 6-MOF showed significant results in the Morris water maze. 6-MOF raised vitamin C levels in the frontal cortex and hippocampus. Serotonin, dopamine, and nor-adrenaline levels were raised in the hippocampus and striatum. It has also imparted region-specific neuroprotection by improving adenosine and its metabolite levels. In silico studies performed using PyRx have shown that the minimum binding energy of 6-MOF with antioxidant enzyme is −7.1 k/cal/mol. The binding energy showed that 6-MOF was successfully docked with an anti-oxidant enzyme. In conclusion, in silico and behavioral studies showed that 6-MOF can be a potential candidate for the treatment of cognitive decline, anxiety, and depression.
Umfang:
Online-Ressource
Fußnote:
Open Access
Archivierung/Langzeitarchivierung gewährleistet
Schlagworte:
serotonin ; dopamine ; noradrenaline ; 6-methoxyflavone ; adenosine
ZLB-Systematik:
Naturwissenschaften
Sonstiges
URN:
urn:nbn:de:101:1-2410261535214.298236181298
Sammlung:
Naturwissenschaften
Sonstiges
Copyright:
CC BY
Zugriffsberechtigung:
Freier Zugang

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