Translational Neuroscience: Trigonelline-treated microglia exosomes mitigates ischemic stroke via inhibiting EIF5A-mediated autophagy

Guo, Liangji; Mei, Xiaoping; Wang, Xiongjiang; Teng, Hongli; Wang, Chenglong; Wei, Jiangcun; Li, Jian

Trigonelline-treated microglia exosomes mitigates ischemic stroke via inhibiting EIF5A-mediated autophagy / Guo, Liangji (CC BY)

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fullscreen : Trigonelline-treated microglia exosomes mitigates ischemic stroke via inhibiting EIF5A-mediated autophagy / Guo, Liangji (CC BY)

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Title:: Translational Neuroscience

Publication:: Berlin Heidelberg: Springer

Note:: Gesehen am 11.08.15; Open Access; Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International; Archivierung/Langzeitarchivierung gewährleistet

Scope:: Online-Ressource

ISSN:: 2081-6936

ZDB-ID:: 2581219-1

VÖBB-Katalog:: 35425620

Keywords:: Zeitschrift

Classification:: Medizin; Naturwissenschaften

Collection:: Medizin; Naturwissenschaften

Copyright:: Rights reserved

Accessibility:: Eingeschränkter Zugang mit Nutzungsbeschränkungen

Title:: Translational Neuroscience

Publication:: Berlin Heidelberg: Springer

Note:: Gesehen am 11.08.15; Open Access; Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International; Archivierung/Langzeitarchivierung gewährleistet

Scope:: Online-Ressource

ISSN:: 2081-6936

ZDB-ID:: 2581219-1

VÖBB-Katalog:: 35425620

Keywords:: Zeitschrift

Classification:: Medizin; Naturwissenschaften

Collection:: Medizin; Naturwissenschaften

Copyright:: Rights reserved

Accessibility:: Eingeschränkter Zugang mit Nutzungsbeschränkungen

Article

Author:: Guo, Liangji; Mei, Xiaoping; Wang, Xiongjiang; Teng, Hongli; Wang, Chenglong; Wei, Jiangcun; Li, Jian

Title:: Trigonelline-treated microglia exosomes mitigates ischemic stroke via inhibiting EIF5A-mediated autophagy

Publication:: Berlin: De Gruyter, 2025

Language:: English

Information:: Objectives: Trigonelline (TRIG) is a neuroprotective agent against various nervous system diseases, including ischemic stroke (IS). Microglia-derived exosomes are crucial mediators in modulating IS damage. Herein, this study intended to investigate the protective mechanism of TRIG in IS by targeting microglia-secreted exosomes. Methods: IS model in vitro was induced by oxygen-glucose deprivation/reoxygenation (OGD/R) in SK-N-SH cells. Exosomes were extracted from TRIG-treated microglia HMC3 cells, and identified by morphology, particle size, and biomarker expression. Cell viability and cytotoxicity were examined using cell counting kit-8 (CCK-8) assay and lactate dehydrogenase (LDH) assay. Enzyme-linked immunosorbent assay (ELISA) and TUNEL assay were performed for assessing inflammation and apoptosis, respectively. The protein expression was tested by Western blotting. Target binding was confirmed by molecular docking and dynamics simulation. Results: TRIG-treated HMC3 cells protected SK-N-SH cells from OGD/R-induced viability inhibition, cytotoxicity, inflammation, and apoptosis. Bilayer membrane structures, diameter size of 120 nm, and expressed exosomal markers were observed in exosomes extracted from TRIG-treated HMC3 cells. Exosomes from TRIG-treated HMC3 cells alleviated OGD/R-induced SK-N-SH cell damage via repressing autophagy. Eukaryotic translation initiation factor 5A (EIF5A) was screened as a target of TRIG associated with exosomes, then molecular docking and dynamic simulation indicated the good binding between TRIG and EIF5A. TRIG-treated HMC3 exosomes suppressed OGD/R-induced IS injury by leading to autophagy inhibition through EIF5A. Conclusions: This study disclosed the role of TRIG targeting microglia exosomes against IS by regulating EIF5A to affect neuronal autophagy, providing further support for the therapeutic potential of TRIG and its molecular mechanism in IS.

Scope:: Online-Ressource

Note:: Open Access; Archivierung/Langzeitarchivierung gewährleistet

Keywords:: trigonelline ; ischemic stroke ; microglia ; exosomes ; eukaryotic translation initiation factor 5A

Classification:: Medizin; Naturwissenschaften; Sonstiges

URN:: urn:nbn:de:101:1-2512310511554.307138543658

Collection:: Medizin; Naturwissenschaften; Sonstiges

Copyright:: CC BY

Accessibility:: Free Access

Author:: Guo, Liangji; Mei, Xiaoping; Wang, Xiongjiang; Teng, Hongli; Wang, Chenglong; Wei, Jiangcun; Li, Jian

Title:: Trigonelline-treated microglia exosomes mitigates ischemic stroke via inhibiting EIF5A-mediated autophagy

Publication:: Berlin: De Gruyter, 2025

Language:: English

Information:: Objectives: Trigonelline (TRIG) is a neuroprotective agent against various nervous system diseases, including ischemic stroke (IS). Microglia-derived exosomes are crucial mediators in modulating IS damage. Herein, this study intended to investigate the protective mechanism of TRIG in IS by targeting microglia-secreted exosomes. Methods: IS model in vitro was induced by oxygen-glucose deprivation/reoxygenation (OGD/R) in SK-N-SH cells. Exosomes were extracted from TRIG-treated microglia HMC3 cells, and identified by morphology, particle size, and biomarker expression. Cell viability and cytotoxicity were examined using cell counting kit-8 (CCK-8) assay and lactate dehydrogenase (LDH) assay. Enzyme-linked immunosorbent assay (ELISA) and TUNEL assay were performed for assessing inflammation and apoptosis, respectively. The protein expression was tested by Western blotting. Target binding was confirmed by molecular docking and dynamics simulation. Results: TRIG-treated HMC3 cells protected SK-N-SH cells from OGD/R-induced viability inhibition, cytotoxicity, inflammation, and apoptosis. Bilayer membrane structures, diameter size of 120 nm, and expressed exosomal markers were observed in exosomes extracted from TRIG-treated HMC3 cells. Exosomes from TRIG-treated HMC3 cells alleviated OGD/R-induced SK-N-SH cell damage via repressing autophagy. Eukaryotic translation initiation factor 5A (EIF5A) was screened as a target of TRIG associated with exosomes, then molecular docking and dynamic simulation indicated the good binding between TRIG and EIF5A. TRIG-treated HMC3 exosomes suppressed OGD/R-induced IS injury by leading to autophagy inhibition through EIF5A. Conclusions: This study disclosed the role of TRIG targeting microglia exosomes against IS by regulating EIF5A to affect neuronal autophagy, providing further support for the therapeutic potential of TRIG and its molecular mechanism in IS.

Scope:: Online-Ressource

Note:: Open Access; Archivierung/Langzeitarchivierung gewährleistet

Keywords:: trigonelline ; ischemic stroke ; microglia ; exosomes ; eukaryotic translation initiation factor 5A

Classification:: Medizin; Naturwissenschaften; Sonstiges

URN:: urn:nbn:de:101:1-2512310511554.307138543658

Collection:: Medizin; Naturwissenschaften; Sonstiges

Copyright:: CC BY

Accessibility:: Free Access

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fullscreen : Trigonelline-treated microglia exosomes mitigates ischemic stroke via inhibiting EIF5A-mediated autophagy / Guo, Liangji (CC BY) Access restriction There is no access restriction for this record. Copyright CC BY: Attribution 4.0 International. You can find more information here.

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fullscreen : Trigonelline-treated microglia exosomes mitigates ischemic stroke via inhibiting EIF5A-mediated autophagy / Guo, Liangji (CC BY)

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